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Disease Free Potato Seed Production through Tissue Culture




  • Introduction

    Potato in Pakistan is cultivated over an area of 103.2 thousand hectares with anDisease_Free_Potato_Seed_Production_through_Tissue_Culture_3 annual production of 1678.5 tonnes. Potato crop is prone to several viral, fungal and bacterial pathogens which cause economic losses up to 70%. On the other hand unavailability of healthy seed is a major problem for achieving maximum yield in Pakistan. There is a need to produce high quality disease free potato seed locally to reduce the import bill.

    Technology Development, Demonstration and Recommendation

    Tissue culture lab at NARC has initiated research on virus free seed potato productionDisease_Free_Potato_Seed_Production_through_Tissue_Culture_2 in Pakistan in early 1980s.Since than this lab is contributing in disease free potato production. The material produced was tested in northern areas on farmers’ fields which were further disseminated in different areas. This technology was then transferred to the private sector and also adopted by the other institutes in Pakistan.  The technology later paved the way for introduction of locally produced potato seed. PARC extended the technology to the provincial research institutes by training manpower and sensitizing the scientific community on the importance of tissue culture technology in potato seed production.

    The Technology

    Tissue culture technology has been developed to produce virus free in vitro plants andDisease_Free_Potato_Seed_Production_through_Tissue_Culture_1 nucleus/basic potato seed. Field demonstration showed high rate of multiplication and increase in the yield which is about 20% more than the normal propagation. This technology is well appreciated by private and public sector.

    Key References

    Hussain, I., Z. Chaudhry, A. Muhammad, R. Asghar, S. M. S. Naqvi and H. Rashid. (2006). Effect of chlorocholine, sucrose and BAP on in vitro tuberization in potato (Solanum tuberosum).  Pakistan Journal of Botany. 38: 275-282.

    Hussain. I., A. Muhammad. H. Rashid. Z. Chaudhry. S. M. Saqlan Naqvi and R. Asghar. (2005). Morphogenic potential of three potato (Solanum tuberosum) cultivars from diverse explants a prerequisite in genetic manipulation).  Pakistan Journal of Botany 37: 889-898.

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